Review



cold facs washing buffer  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Miltenyi Biotec cold facs washing buffer
    Cold Facs Washing Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+washing+buffer/facs+buf/pmc09844005-48-13-16
    Average 90 stars, based on 1 article reviews
    cold facs washing buffer - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    FACS:

    Article Title: Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT‐II T Cell Activation Conferring Tumor Protection in Mice
    Article Snippet: .. Cells were collected in FACS tubes and washed three times with cold FACS washing buffer (Miltenyi Biotec, Germany). .. For live/dead discrimination and T cell analysis, cells were stained with activated Caspase 3/7 detection reagent (Thermo Scientific, USA) and fc block (BioLegend, UK) at room temperature for 10 min followed by incubation with fluorescently conjugated monoclonal antibodies targeting CD62L (PE‐Dazzle, clone MEL‐14), CD44 (PerCP‐Cy5.5, clone IM7), CD4 (PE‐Cy7, clone L3T4), CD25 (APC, clone PC61), CD3 (Alexa Fluor 700, clone 17A2), CD69 (brilliant violet 421, clone H1.2F3), and CD8 (brilliant violet 510, clone 53–6.7) (all BioLegend, UK) for 30 min at 4 °C.

    Article Title: Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling
    Article Snippet: Briefly, cells were stained seven days after first NMP exposure with 2 μM Calcein-AM, 1 μM of propidium iodide (PI), and 5 μM of Hoechst 33342 (Life Technologies, USA), and imaged using fluorescence microscopy (Axio Observer Z.1; Zeiss, Germany). .. Additionally, cells were collected in FACS tubes and washed three times with cold FACS washing buffer (Miltenyi Biotec, Germany). .. Cells were stained with caspase 3/7 detection reagent (ThermoFisher, Germany) and 4′,6-diamidine-2-phenylindole (DAPI, final concentration 1 μM; BioLegend, The Netherlands) at 37 °C for 30 min. After washing with cold FACS washing buffer, samples were measured using flow cytometry (CytoFLEX S/LX; Beckman-Coulter, Germany) and analyzed using Kaluza software 2.1 (Beckman-Coulter, USA).



    Similar Products

    cold facs washing buffer  (Miltenyi Biotec)
    90
    Miltenyi Biotec cold facs washing buffer
    Cold Facs Washing Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+washing+buffer/facs+buf/pmc09844005-48-13-16
    Average 90 stars, based on 1 article reviews
    cold facs washing buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cold facs wash buffer  (Thermo Fisher)
    99
    Thermo Fisher cold facs wash buffer
    Cold Facs Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+washing+buffer/Bovine+Serum+Albumin/pm41309138-110-7-26
    Average 99 stars, based on 1 article reviews
    cold facs wash buffer - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    cold facs wash buffer  (Becton Dickinson)
    90
    Becton Dickinson cold facs wash buffer
    Cold Facs Wash Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+washing+buffer/facs+buffer/pmc09125252-95-5-8
    Average 90 stars, based on 1 article reviews
    cold facs wash buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cold facs wash buffer  (Spherotech inc)
    90
    Spherotech inc cold facs wash buffer
    Cold Facs Wash Buffer, supplied by Spherotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+washing+buffer/cold+facs+wash+buffer/pm32041902-182-13-31
    Average 90 stars, based on 1 article reviews
    cold facs wash buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    ice-cold facs washing buffer  (Becton Dickinson)
    90
    Becton Dickinson ice-cold facs washing buffer
    A. Phagocytosis of C. albicans by WT and Gsr−/− neutrophils. Purified BM neutrophils were incubated with pHrodo red-tagged C. albicans (MOI=10) at 0°C or 37°C for 30 min, and then labeled with Ly6G–Pacific Blue on ice for 20 min. Cells were subsequently washed twice with cold <t>FACS</t> buffer and analyzed by <t>flow</t> <t>cytometry.</t> Ly6G+ neutrophils cells were analyzed for the presence of pHrodo red-tagged C. albicans. Solid lines depict Gsr+/+ and dashed lines depict Gsr−/− neutrophils. B. Fungal killing assays. Serum-opsonized C. albicans yeast were incubated with either purified BM neutrophils or medium for 60 min at an MOI of 10:1, and then the neutrophils were lyzed by hypo-osmolarity. The mixtures were then serially diluted, and plated onto YPD agar plates to culture at 37°C for approximately 24 h. Viable C. albicans cells were scored by counting the colonies formed on the YPD plates. The percentage of killing was calculated using the medium-treated C. albicans as references. Values are means ± SE. *, p<0.05 (t-test, n=3).
    Ice Cold Facs Washing Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cold+facs+washing+buffer/facs+buffer/pmc06783371-199-7-17
    Average 90 stars, based on 1 article reviews
    ice-cold facs washing buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Phagocytosis of C. albicans by WT and Gsr−/− neutrophils. Purified BM neutrophils were incubated with pHrodo red-tagged C. albicans (MOI=10) at 0°C or 37°C for 30 min, and then labeled with Ly6G–Pacific Blue on ice for 20 min. Cells were subsequently washed twice with cold FACS buffer and analyzed by flow cytometry. Ly6G+ neutrophils cells were analyzed for the presence of pHrodo red-tagged C. albicans. Solid lines depict Gsr+/+ and dashed lines depict Gsr−/− neutrophils. B. Fungal killing assays. Serum-opsonized C. albicans yeast were incubated with either purified BM neutrophils or medium for 60 min at an MOI of 10:1, and then the neutrophils were lyzed by hypo-osmolarity. The mixtures were then serially diluted, and plated onto YPD agar plates to culture at 37°C for approximately 24 h. Viable C. albicans cells were scored by counting the colonies formed on the YPD plates. The percentage of killing was calculated using the medium-treated C. albicans as references. Values are means ± SE. *, p<0.05 (t-test, n=3).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Glutathione Reductase Promotes Fungal Clearance and Suppresses Inflammation during Systemic Candida albicans Infection in Mice

    doi: 10.4049/jimmunol.1701686

    Figure Lengend Snippet: A. Phagocytosis of C. albicans by WT and Gsr−/− neutrophils. Purified BM neutrophils were incubated with pHrodo red-tagged C. albicans (MOI=10) at 0°C or 37°C for 30 min, and then labeled with Ly6G–Pacific Blue on ice for 20 min. Cells were subsequently washed twice with cold FACS buffer and analyzed by flow cytometry. Ly6G+ neutrophils cells were analyzed for the presence of pHrodo red-tagged C. albicans. Solid lines depict Gsr+/+ and dashed lines depict Gsr−/− neutrophils. B. Fungal killing assays. Serum-opsonized C. albicans yeast were incubated with either purified BM neutrophils or medium for 60 min at an MOI of 10:1, and then the neutrophils were lyzed by hypo-osmolarity. The mixtures were then serially diluted, and plated onto YPD agar plates to culture at 37°C for approximately 24 h. Viable C. albicans cells were scored by counting the colonies formed on the YPD plates. The percentage of killing was calculated using the medium-treated C. albicans as references. Values are means ± SE. *, p<0.05 (t-test, n=3).

    Article Snippet: Subsequently, the cells were washed twice with ice-cold FACS washing buffer prior to flow cytometry on a BD LSR II flow cytometer (BD Biosciences).

    Techniques: Purification, Incubation, Labeling, Flow Cytometry

    A. C. albicans-induced cytokine production by WT and Gsr−/− macrophages. WT and Gsr−/− BMDM were stimulated with heat-killed C. albicans (MOI=30, 100) for 24 or 48 h, and cytokines in the medium were quantified by ELISA. *, p<0.05 (t-test, n=4). B. Phagocytosis of C. albicans by WT and Gsr−/− BMDM. WT and Gsr−/− BMDM were incubated with pHrodo red-tagged C. albicans (MOI= 3 or 10) at 37°C for 30 min, and then labeled with Pacific blue-labelled anti-mouse F4/80 on ice for 10 min. Cells were subsequently washed twice with cold FACS buffer and analyzed by flow cytometry. F4/80+ BMDM were analyzed for the presence of pHrodo red-tagged C. albicans. Solid lines depict Gsr+/+ and dashed lines depict Gsr−/− cells.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Glutathione Reductase Promotes Fungal Clearance and Suppresses Inflammation during Systemic Candida albicans Infection in Mice

    doi: 10.4049/jimmunol.1701686

    Figure Lengend Snippet: A. C. albicans-induced cytokine production by WT and Gsr−/− macrophages. WT and Gsr−/− BMDM were stimulated with heat-killed C. albicans (MOI=30, 100) for 24 or 48 h, and cytokines in the medium were quantified by ELISA. *, p<0.05 (t-test, n=4). B. Phagocytosis of C. albicans by WT and Gsr−/− BMDM. WT and Gsr−/− BMDM were incubated with pHrodo red-tagged C. albicans (MOI= 3 or 10) at 37°C for 30 min, and then labeled with Pacific blue-labelled anti-mouse F4/80 on ice for 10 min. Cells were subsequently washed twice with cold FACS buffer and analyzed by flow cytometry. F4/80+ BMDM were analyzed for the presence of pHrodo red-tagged C. albicans. Solid lines depict Gsr+/+ and dashed lines depict Gsr−/− cells.

    Article Snippet: Subsequently, the cells were washed twice with ice-cold FACS washing buffer prior to flow cytometry on a BD LSR II flow cytometer (BD Biosciences).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Labeling, Flow Cytometry